Recombinant Human Hepatocyte Growth Factor (HGF) (Active)
Bioactive Recombinant HGF Protein for Cell Signaling and Cancer Research
PRODUCT OVERVIEW
Recombinant Human Hepatocyte Growth Factor (HGF) is an active protein involved in the regulation of cell growth, motility, morphogenesis, and tissue development. Also known as Scatter Factor (SF), HGF is a multifunctional cytokine that acts primarily on epithelial cells through interaction with the c-Met receptor.
This recombinant human HGF protein is produced using a mammalian cell expression system and features a C-terminal 6xHis tag. It exhibits biological activity and is suitable for research involving HGF/c-Met signaling, cellular proliferation, angiogenesis, cancer biology, and tissue regeneration.
PRODUCT FEATURES
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Active HGF protein for investigating c-Met signaling, cell proliferation, migration, and tissue development. |
BIOLOGICAL RELEVANCE
Hepatocyte Growth Factor (HGF), also called Scatter Factor, is a multifunctional growth factor secreted primarily by mesenchymal cells. It binds to the MET receptor tyrosine kinase, promoting receptor dimerization and activation of downstream signaling pathways.
HGF/c-Met signaling contributes to cellular proliferation, survival, migration, and morphogenesis. This pathway is particularly relevant to studies of liver regeneration, angiogenesis, tumor development, tissue repair, and interactions between epithelial and mesenchymal cells.
RESEARCH APPLICATIONS
- HGF/c-Met receptor signaling pathway research
- Cancer biology and tumor progression studies
- Cell proliferation and migration assays
- Angiogenesis and morphogenesis research
- Liver regeneration and hepatocyte biology
- Tissue repair and regenerative medicine research
PRODUCT SPECIFICATIONS
| Product Name | Recombinant Human Hepatocyte Growth Factor (HGF) (Active) |
| Catalog Number | 0399-CSB-AP003681HU-01 |
| Size | 50 µg |
| Alternative Names | Hepatocyte Growth Factor, HPTA, HGF, SF, Scatter Factor, Hepatopoietin-A |
| Abbreviation | Recombinant Human HGF Protein (Active) |
| Gene Name | HGF |
| UniProt ID | P14210 |
| Expression Region | 32–728 aa |
| Organism | Homo sapiens (Human) |
| Protein Length | Full Length of Mature Protein |
| Molecular Weight | 79.7 kDa |
| Tag | C-terminal 6xHis-tagged |
| Type | Active Protein & In Stock Protein |
| Source | Mammalian Cell |
| Field of Research | Cancer Research |
| Form | Lyophilized Powder |
| Dry Ice | No |
| Hazardous | No |
PURITY, ENDOTOXIN AND BIOACTIVITY
| Purity | Greater than 95% as determined by SDS-PAGE |
| Endotoxin | Less than 1.0 EU/µg as determined by LAL method |
| Activity | Yes – Biologically Active |
| Bioactivity / ED50 | 0.3–1.5 ng/mL, determined by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells |
BUFFER AND RECONSTITUTION
Buffer Composition
Lyophilized from a 0.2 µm filtered solution containing 20 mM Tris, 500 mM NaCl, 3% Trehalose, and 0.02% Tween 80, pH 8.0.
Reconstitution Instructions
- Briefly centrifuge the vial before opening to bring the lyophilized contents to the bottom.
- Reconstitute the protein in deionized sterile water to a concentration of 0.1–1.0 mg/mL.
- For long-term storage, the recommended final glycerol concentration is 5–50%, with 50% used as the default reference concentration.
- Aliquot the reconstituted protein and store at -20°C or -80°C.
BIOLOGICAL FUNCTION
HGF acts as a potent mitogen for mature parenchymal hepatocytes and functions as a hepatotrophic growth factor for a broad range of tissues and cell types. It is the activating ligand of the MET receptor tyrosine kinase, binding to the receptor and promoting its dimerization to initiate intracellular signaling.
STORAGE AND STABILITY
The shelf life depends on several factors, including storage state, buffer composition, temperature, and protein stability.
| Liquid Form | 6 months at -20°C / -80°C |
| Lyophilized Form | 12 months at -20°C / -80°C |
For research use only. Not intended for diagnostic or therapeutic applications. Please refer to the product documentation for complete handling, reconstitution, and storage instructions.